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Application and Use of Basement Membrane Matrix (Matrigel)
Basement membrane matrix, commonly known as Matrigel, is a naturally derived extracellular matrix extracted from EHS mouse tumors. It contains key components such as laminin, type IV collagen, nestin, heparin-binding growth factors, and matrix metalloproteinases. At room temperature, Matrigel polymerizes into a biologically active three-dimensional structure that closely resembles the in vivo basement membrane in terms of composition, physical properties, and functional characteristics. This makes it an ideal support for cell culture, differentiation, migration, infection studies, and gene expression analysis. Shanghai Yuanmu Biochemical Reagent Supplier offers high-quality Matrigel for research purposes.
**How to Use Matrigel:** 1. **Coating Cells:** You can directly coat cells without dilution, with coating thickness ranging from 0.5 mm (thin layer) to 1.0 mm (thick layer). Alternatively, Matrigel can be diluted in serum-free medium and used to coat the surface of culture vessels. The concentration should be adjusted based on the cell type and experimental purpose. 2. **Temperature Control:** Matrigel should be applied at temperatures above 10°C. During handling, it should remain on ice. All materials that come into contact with Matrigel—such as culture vessels, pipette tips, and dispensing tubes—must be pre-cooled to prevent premature gelation. 3. **Color Changes:** Matrigel may change color from light yellow to deep red due to the interaction of phenol red with bicarbonate and CO₂. This color variation will diminish after equilibration with 5% CO₂. After freezing and thawing, gently shake the bottle to ensure even distribution. All procedures must be performed under sterile conditions. Before use, the bottle cap should be wiped with 70% ethanol and allowed to dry. A pre-cooled pipette should be used to maintain homogeneity. 4. **Storage and Handling:** Matrigel can be stored at 4°C overnight to dissolve. Once gelled, it can be re-liquified by keeping it at 4°C for 24–48 hours.
**Applications of Matrigel:** The three-dimensional matrix formed by Matrigel supports the adhesion and differentiation of various cell types, including epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, and hair follicle cells. It also influences protein expression in mammary epithelial cells, promotes peripheral nerve regeneration, and supports the differentiation of bovine oviductal epithelial cells. Different formulations of Matrigel are available to meet specific experimental needs. Whether you're conducting basic research or advanced cell biology studies, Matrigel provides a reliable and versatile platform for in vitro experimentation.
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