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Application and Use of Base Rubber
Basal glue, also known as Matrigel, is a basement membrane matrix extracted from EHS mouse tumors. It is rich in extracellular matrix proteins such as laminin, type IV collagen, nestin, heparin-binding glycoprotein, growth factors, and matrix metalloproteinases. At room temperature, it polymerizes into a biologically active three-dimensional matrix that closely mimics the structure, composition, physical properties, and functions of the in vivo cell basement membrane. This makes it highly suitable for in vitro cell culture and differentiation studies, and it can be used to investigate cell morphology, biochemical functions, migration, infection, and gene expression. Shanghai Yuanmu Biochemical Reagent Supplier offers high-quality Matrigel for research purposes. Here’s how to use it effectively: 1. You can directly coat cells without dilution, with a coating thickness of either 0.5 mm (thin layer) or 1.0 mm (thick layer). Alternatively, you can dilute Matrigel in serum-free medium and coat the surface of the culture vessel. The appropriate concentration depends on the cell type and experimental purpose. 2. Matrigel should be applied at temperatures above 10°C. During handling, always keep it on ice. All cell culture vessels, pipette tips, and dispensing tubes that come into contact with Matrigel must be pre-cooled. 3. The Matrigel may change color, from light yellow to deep red, due to the interaction of phenol red with bicarbonate and CO₂. This color variation will diminish after equilibration with 5% CO₂. After freezing and thawing, gently shake the bottle to ensure even distribution. All procedures should be performed in a sterile environment. Wipe the reagent bottle cap with 70% ethanol and allow it to air dry. Use a pre-cooled pipette to maintain homogeneity of the Matrigel. 4. To dissolve Matrigel, place it in the refrigerator at 4°C overnight. Once gelled, it can be re-liquified by keeping it at 4°C for 24–48 hours. Applications of Base Glue: Matrigel serves as an effective three-dimensional culture medium that promotes adhesion and differentiation of various cell types, including epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, and hair follicle cells. It also influences protein expression in mammary epithelial cells, supports peripheral nerve regeneration, and aids in the differentiation of bovine oviductal epithelial cells. Different formulations of Matrigel are available to meet diverse experimental needs, making it a versatile tool in biological research.
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